Fluorescent labelling across the spectrum and 'light up' peptides from CRB

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Spectrum Peptides: Full Spectra

Cambridge Research Biochemicals offers the full emission spectra of fluorescent labelled peptides

Chromis dyes: New fluorescent labels

The CHROMIS product line is a selection of the newest fluorescent labels available today.

Dye Selector: Choosing the best Dye

Our dye selector will take you through a step by step process to help you choose the best dye.

In a recent Nature Biotechnology paper, authors have found potential for fluorescent peptides to illuminate nerves during surgery. A frightening complication of surgery can result in paralysis, chronic pain from unintended peripheral nerve damage. To visualise and avoid these nerves has always been challenging. For example, if the nerves are invaded by a tumour, or, if surgery is required in the setting of trauma or infection, the affected nerves might not look as they normally would, or their location may be distorted. Scientists at the University of California San Diego (UCSD) have developed fluorescent peptides; referring to these as “Light-up Peptides” to visualise nerves during surgeries.

Convenience:

CRB has the most comprehensive range available of
licenced and free-to-use activated fluorescent dyes for labelling peptides.

Advice:

CRB brings thirty years of experience of labelling peptides with fluorescent dyes and will advise on the synthesis strategy for most efficient use of dye and hence cost-effectiveness.

Range:

CRB provides a dye selector tool and a technical support service to best match dye choice with intended application and excitation source.

Licenced Proprietary dye range:

	Cy™ dyes from GE-Amersham Biosciences
	Alexa Fluor^® dyes from with Life Technologies-Molecular Probes
	ATTO dyes from Atto-Tec
	Black Hole Quenchers from Biosearch Technologies

Innovation:

CRB can now offer a custom dye design and synthesis option for development of dyes for specific projects which need to vary structure, reactivity or charge of dyes including new derivatives.

Versatile:

Leave it to the experts, contact CRB Discovery for all your fluorescent labelling needs be it peptide, protein, antibody or small molecule.

Cyanagen are CRB’s latest supplier of bright, cost-effective and non-proprietary (free to use) fluorescent dyes. The CHROMIS product line is a selection of the newest fluorescent labels available today. Dye structures were carefully chosen to provide top-notch brightness performances, improving bioanalytical assays to the highest level.

Eight different basic structures span the full working range of wavelength, starting from 400 nm up to 870 nm. Each fluorescent compound is available with many different reactive moieties while retaining the photophysical features of the parent dye.

Furthermore, a completely new level of chemistry has been developed to provide the same fluorescent label with different charge values (anionic, cationic, zwitterionic and neutral).

References

Olivier A. Pierrat, Kvido Strisovsky, Yonka Christova, Jonathan Large, Keith Ansell, Nathalie Bouloc, Ela Smiljanic, Matthew Freeman

Monocyclic β-Lactams Are Selective, Mechanism-Based Inhibitors of Rhomboid Intramembrane Proteases

ACS Chemical Biology In Publication

Link: http://pubs.acs.org/doi/full/10.1021/cb100314y

Webb S.E.D, Roberts S.K, Needham S.R, Tynan C.J, Rolfe D.J, Winn M.D.
Clarke D.T, Barraclough R, Martin-Fernandez M.L

Single-Molecule Imaging and Fluorescence Lifetime Imaging Microscopy Show Different Structures for High- and Low-Affinity Epidermal Growth Factor Receptors in A431 Cells

Biophysical Journal, 2008. 94(3), 803-819

Link: http://www.cell.com/biophysj/abstract/S0006-3495(08)70680-5

Our dye selector will take you through a step by step process to help you choose the best dye for your application.

With licenses to supply materials labelled with GE Healthcare’s Cy™ Dyes and Life Technologies - Molecular Probes’ Alexa Fluor^® dyes range, we offer the most comprehensive custom labelling service for fluorescent peptides.

A range of red, green and blue dyes are available to support a wide variety of discovery technologies such as FRET or multiplex assays and fluorescence microscopy. Our innovative online dye selector provides a list of suitable dyes matching your wavelength and provides the necessary technical data to enable choice of dye fit for purpose. Alternatively you can talk to us directly for advice on your specific application.

Should you require a quotation from any of our custom services, we have a clear cut and easy system that enables you to do so. Follow these three simple steps:

Go to: www.crbdiscovery.com/quotations

Simply click on the relevant tab and register your details.

Once issued with a password you can then log on to our quotation system using your customer account log-in and a quotation will be issued within 24 hours.

At Cambridge Research Biochemicals we have a fast-track quotation system that tailors your requirements, gives you all the information you need and a specific price within 24 hours. You are also given a personal contact so that if you have any further questions you can directly contact one of our approachable representatives who has the technical knowledge to assist you.