Can't view this email? View it online instead Contact us   Case Study Stapled Peptides - the α-helix stabilisation of peptides PURE™ Peptides CRB offers a range of peptide service categories providing every type of peptide request possible RSC Protein and Peptide Science Group Appointment of CRB's Laurent Caron and forthcoming events     There has been a lot of discussion around α-helix stabilisation of peptides in the past few years and for good reasons: because peptides in their native form usually exhibit little structure compared to proteins, they show limited stability to proteolysis and poor membrane penetration. Increasing α-helicity of peptides has been shown to increase protease stability, cell-permeability and binding affinity to their target. One man, Gregory Verdine (Harvard University), pioneered the development of stapled peptides and paved the way to this novel class of synthetic peptides via hydrocarbon stapling. In a recent chapter in Methods in Enzymology, the pre-eminent researcher explains the ins and outs of hydrocarbon stapling. http://www.ncbi.nlm.nih.gov/pubmed/22230563 Consultative Partnering CRB works extremely closely with its customers to ensure that every peptide produced is appropriate for its intended application and fulfils the customer’s requirements. To achieve this, each peptide project is carefully screened by our technical staff, and where appropriate we will advise on changes to the design of the peptides and the synthesis strategy. Extensive Capability Routine peptides - 1 mg-10 g Single peptides to large arrays Cyclic peptides - disulphide and head-to-tail, side-chain to side-chain or backbone lactam bridged Post-translational modifications - phosphorylated, sulphated, methylated & glycosylated peptides C-terminal modifications - secondary amides, alcohols, aldehydes, esters, AMCs, pNAs Peptide Applications Within the field of biology chemically synthesised peptides are a very powerful research tool. Peptides can be used in wide-ranging applications both in-vitro and in-vivo to investigate biological pathways and hence are an integral part of the drug discovery process. Some typical applications our peptides are used for are: Production of anti-protein antibodies where a peptide is used as a surrogate for the protein Enzyme activity studies in which a peptide substrate is used to investigate actions of enzymes such as kinases which are critically important in cancer research Binding studies in which a fluorescently labelled or radiolabelled peptide is used to study binding of the peptide ligand to proteins Study of post-translational modifications such as phosphorylation and methylation where these modifications can be chemically included within a peptide Quality Purity: >95% by HPLC Peptide identity confirmed by mass spectrometry (ESI or MALDI-TOF) In-process control of the synthesis of complex peptides by mass spectrometry and HPLC monitoring Customer Support At CRB we pride ourselves on the quality of our peptides and our customer focussed service: Detailed consultation on peptide design Superior building block and synthesis strategies Custom chemical synthesis of unusual reagents Regular progress updates Custom aliquotting upon request Excess peptide retained in stock Peptide Inventory dating back to 1980 CASE STUDY Arg-Ser-Gly-Pro-Pro-Gly-[S-α-(4'-pentenyl-alanine)]-Gln-Gly-Arg-[S-α-(4'-pentenyl-alanine)]-Gln-Arg-Leu-Leu-Gln-Ala-Ser-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr-Met-NH2   Helix stabilisation by cross-linking is a relatively new approach enabling the design of highly selective peptides. To minimise the amount of expensive Verdine building blocks used for Ring-closing metathesis, 40μmol of peptidyl-resin was treated with Grubbs catalyst. Careful control of the quality of the peptide before and during stapling enabled the preparation of stapled Orexin B in excellent yield. Dr Laurent Caron (Technical Manager, Cambridge Research Biochemicals) has been appointed membership of the Royal Society of Chemistry Protein and Peptide Science group and will attend his first committee meeting on 20th September, 2012 at Burlington House, London (home to five learned societies including the RSC). The RSC Protein and Peptide Science group originally founded in 1973 has a strong historic basis in Peptide chemistry. The interests of the Group have developed in recent years to cover: Protein structure determination, structure prediction, molecular modelling and dynamics, Protein separation and characterisation, Protein biosynthesis, Protein expression, modification and targeting, combinatorial synthesis, Protein design and engineering, drug-Protein and Protein-Protein interactions and Protein structure-function. The Group is actively engaged in the organisation of meetings to promote Peptide and Protein Science, and in representing it’s members' interests in the wider scientific community, in government, at Research Councils, and at grant giving bodies such as the Wellcome Trust. The Group attempts to influence positively the way in which science is taught in schools, colleges and universities. "I am delighted to join the RSC Protein and Peptide Science group, it represents a great honour for me and I hope that my input from an industry perspective will benefit the group and all Peptide enthusiasts worldwide." Dr Laurent Caron of Cambridge Research Biochemicals (CRB) Dr Laurent Caron Technical Manager     "The appointment of Dr Laurent Caron was unanimously agreed and it is good news to have a representative from the second oldest global Peptide company, CRB, on board."   Kevin Howland (Membership Secretary) Manager of Biomolecular Science Facility, University of Kent Join the RSC Protein and Peptide Science Interest Group Unmodified, K-acetylated and K-monomethylated versions of TYETFKSIMKKSPFSGPTDP-acid Functional Interplay between Acetylation and Methylation of the Re1A Subunit of NF-kB X-D. Yang, E. Tajkhorshid &a,p; L-F. Chen. University of Illinois Molecular & Cellular Biology 2010, 30, 9, 2170-2180 MEVGWYRSPFSRVVHLYRNGK-acid Myelin Oligodendrocyte Peptide (MOG 35-55) Modulation of experimental autoimmune encephalomyletis by endogenous Annexin A1 N. Paschalidis, A. J. Iqbal, F. Maione, E. G. Wood, M. Perreti, R. J. Flower & F. D'Acquisto. Queen Mary University of London Journal of Neuroinflammation 2009, 6:33 Multiple peptides with N-terminal biotin up to 35 residues in length Two modules in the BRC repeats of BRCA2 mediate structural and functional interactions with the RAD51 recombinase E. Rajendra & A. R. Venkitaraman. Medical Research Council Cancer Cell Unit Nucleic Acids Research, 2009, 1-15 Should you require a quotation from any of our custom services, we have a clear cut and easy system that enables you to do so.   At Cambridge Research Biochemicals we have a fast-track quotation system that tailors your requirements, gives you all the information you need and a specific price within 24 hours. You are also given a personal contact so that if you have any further questions you can directly contact one of our approachable representatives who has the technical knowledge to assist you.   Go to: www.crbdiscovery.com/quotations         Click on the relevant tab and register your details.         Once issued with a password log on to our quotation system using your customer account log-in and a quotation will be issued within 24 hours.