World Renowned capability and twenty nine years pedigree

  1. Peptides
  2. PNA
  3. Fluorescent Dyes
  4. Immunological
  1. Chromis D+A
  2. Chromis DGE
  3. Synthetic Haptens

Chromis DGE kits

Cyanagen has extended its D+A technology to CHROMIS DGE Minimal Labelling Kits, specifically optimized for protein detection in 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE). Minimal labelling requires a minimized concentration of dye in order to label each protein with a single dye molecule. Labelling occurs by forming an amide bond between the ε-amino group of lysine in proteins and the activated dye. For a complete Minimal Labelling DIGE analysis, Cyanagen has developed the products below.

The fluorescent dyes in the CHROMIS DIGE Minimal Labelling Kits are designed to be:

  • Charge-matched: a positive charge on each dye replaces the one lost from lysine upon formation of the amide bond, leaving the total charge on the protein unchanged
  • Size-matched: all dyes have similar molecular weight to minimize differences in protein migration after labelling
  • pH insensitive: the fluorescent signal is not influenced by pH over a wide range
Name
λex
λem
 CHROMIS 3X DGE - Minimal Labelling Kit for DGE

see entries for appropriate dyes 

see entries for appropriate dyes 
 CHROMIS 490 DGE - Minimal Labelling Kit for DGE

484 nm 

498 nm 
 CHROMIS 550 DGE - Minimal Labelling Kit for DGE

548 nm 

567 nm 
 CHROMIS 645 DGE - Minimal Labelling Kit for DGE

641 nm 

663 nm