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  2. PNA
  3. Fluorescent Dyes
  4. Immunological
  1. Chromis D+A
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Westar: Enhanced Chemiluminescence Detection (ECL) Kits with Improved Sensivity and Stability

Westar products are a range of complete, ready-to-use kits for the horseradish peroxidase-based chemiluminescence detection of immobilised proteins (Western Blot) and immobilised nucleic acids (Southern and Northern Blot).

WESTAR kits offer the following advantages over conventional ECL reagents:

  • Outstanding Sensitivity: Kits range from 8 to 150 times more sensitive than those based on phenolic chemiluminescence enhancers
  • Cost Effective: use 50- to 100-fold less antibody than with conventional ECL
  • Long Signal Duration: 6h to 24 h signal duration
  • Versatile Signal Detection: The chemiluminescent signal used in Westar substrates can be detected either by film or by digital cameras based on CCD sensors
  • Safer: None of the components used in Westar formulations has been reported to be hazardous to human health
  • More Stable: The shelf life of all Westar stock reagents exceeds 1 year at 2-8 °C. The working solutions, obtained by mixing the two components of the kits in a one-to-one ratio, are stable at room temperature for either 24 hours (Westar ETA C and XT) or 8 hours (Westar Supernova)
Kits for Blots
Description
Intensity
Sensitivity
Signal Duration
Suggested Antibody Dilution Range
Westar ETA C High intensity light output - at least 20 times more intense than standard ECL based on phenolic enhancers. Economical: less costly than any other chemiluminescent substrates. 10-13 g 6 h> Primary: 1:1,000-1:15,000; Secondary: 1:25,000-1:150,000

Westar XT

 24 h signal duration allows sufficient time to make multiple exposures for publication-quality blots. 20 times more sensitive than standard ECL using phenolic enhancers. Dilute expensive antibodies up to 50 times further than with standard chemiluminescent substrates. 10-14 g 24 h Primary: 1:1,000-1:50,000; Secondary: 1:50,000-1:250,000
Westar Supernova The most sensitive chemiluminescent substrate available - at least 150 times more intense than standard substrates. Dilute expensive antibodies up to 100 times further than with standard chemiluminescent substrates. 10-15 g 8 h Primary: 1:5,000-1:100,000; Secondary: 1:100,000-1:500,000

Westar kits are also available for ELISA assays. These offer excellent signal intensity and stability compared to conventional methods.

Kits for ELISA
Description
Sensitivity
Signal Stability
Working Solution Stability
Westar ETA C 10-13 - 10-14 g 30 mins 24 h
Westar Supernova 10-15 g 30 mins 6 h