World Renowned capability and twenty nine years pedigree

Services: Specialist peptide synthesis & antibody production from CRB

  1. Peptide Synthesis
  2. Antibody Production
  3. Labelling
  4. PNA Synthesis

Infinity Antibodies

Our Monoclonal Antibody service is a way of generating antibodies in perpetuity. Once successfully made these antibodies can be duplicated time and time again to infinity.

This one-stop expedient service is brought to you due to the partnership between two long-lasting specialists. CRB provides the chemical insight and customer support by providing the peptide antigen and project management whilst BBT (Babraham Bioscience Technologies) generates the hybridoma and produces the antibody.

We offer a comprehensive Monoclonal antibody service which can be progressed or terminated at each stage dependent upon success of the following steps:

  1. Phase 0:  Antigenic prediction – peptide synthesis – carrier conjugation
  2. Phase 1:  Immunisation and screening
  3. Phase 2:  Fusion and cloning
  4. Phase 3:  Sub-cloning and delivery of antibody
  5. Phase 4:  Scale-up and purification

Monoclonals offer many advantages over traditional polyclonals:

  1. Immortal cell line - antibody available in perpertuity
  2. Defined specificity
  3. Low non-specific activity
  4. Ideal for low abundance antibodies, such as IgM
  5. In-process assay optimisation
  6. Ownership of cell line
  7. Offers reproducibility & traceability
Project Design
  1. Expert advice on the vital first step of antigen selection from either your target protein or peptide sequence
  2. Design of screening strategy to select for clones against specific PTMs or protein regions
  3. Choice of appropriate species (mouse, rat, rabbit)
Optimised Strategy
  1. Extended immunisations to achieve optimum titre prior to cloning
  2. Development of screening assay to identify best clones
  3. Multiple sub-cloning to capture stable cell lines
  4. Scaleable production and purification capacity Customer support
  5. Regular progress updates
  6. Defined check points for project progression
  7. Written reports available at end of each phase
  8. Back-up freezing of sub-clones and final cell line
Customer support
  • Regular progress updates
  • Defined check points for project progression
  • Written reports available at end of each phase
  • Back-up freezing of sub-clones and final cell line
Customer account