Services: Specialist peptide synthesis %26 antibody production from CRB
PEAK™ Peptides
CRB specialises in the rapid synthesis of peptides containing stable isotope labelled amino acids.
By using stable-labelled versions of tryptic peptides as internal standards, it is possible to directly quantify protein levels in a digested sample via mass spectrometry.
Quality
Critical to the accuracy of this technique is the quality of the stable-labelled peptide. CRB’s custom stable–labelled peptides are offered:
- Critical to the accuracy of this technique is the quality of the stable-labelled peptide.
- >95% pure by HPLC
- analysed for net peptide content.
Post-translational modifications
For the quantitation of post-translational modifications, CRB can also offer stable-labelled peptides containing:
- phosphoTyr, phosphoSer, phosphoThr
- sulfoTyr
- methylated Lys and Arg
Other proteolysis standards
Isotopically labelled versions of peptides derived from proteolysis by other enzymes, such as Lys-C, Glu-C, can also be produced.
The stable-labelled amino acids most commonly used in this technique are shown in Table 1. These amino acids provide the greatest shift in the molecular weight for the least cost; however, heavy atom versions of other amino acids are available upon request.
Delivery
Streamlined operation allows delivery in 2 - 3 weeks. Most common stable-labelled amino acid building blocks are kept in stock to ensure rapid turn-around.
Technical support
CRB are happy to advise on the design of stable-labelled peptides. CRB can also assist with pre-concentration techniques to significantly reduce the limit ofdetection via the production of antibody pre-concentration columns.
