Technical: Clear & informative notes on specialist areas
Antibodies
Outline of the Polyclonal Antibody process
Peptide Antigen Selection
- Ability to work with full protein/dna or truncated sequence
- Free design of antigenic peptides
- Expert advice on favourable positions within defined regions
- Conjugation strategy always considered
Peptide Synthesis
- High Purity >80% or >95%
- Peptides from the inventors
- Peptides manufactured and analysed on site
- Rigorous Quality Control by HPLC and Mass
Conjugation
- Optimised chemistry
- Range of carriers available
- Peptide-carrier ratio measured
Immunisation
- Project started 2 weeks from order
- Proven protocol for guaranteed anti-peptide response
- Test samples available in six weeks
- Online tracking for ease of updates
- Project completion upon clients instruction
Screening
- Optimised anti-peptide ELISA system
- Easy to view results uploaded to customer web area
- Direct communication with our scientists
- Advice on direction of projects
Purification
- Peptide Affinity chromatography for specific anti-peptide antibodies
- Protein A or G purification
- Pre-immune purified control
- Phospho-specific pTyr, pSer, pThr antibodies
- Depletion strategies for unwanted antibodies or controls
Modifications
- F(ab) or F(Ab')2 fragmentation
- Labelling of primary antibodies with HRP, Biotin, FITC, Cy and Alexa dyes
Additional Services
- Western blotting for anti-protein response
- Aliquotting, labelling and storage
