Tryptic peptide selection

Tryptic peptide selection

Guidelines for stable-labelled tryptic peptide selection

  • 8-20 residues: 800 — 2000 MU
  • Contain Leu, Val, Pro, Phe
  • Avoid sequences containing Met or Cys. If Cys must to be included, the Cys can be introduced as the Cys(CH2NHCOCH3) derivative, to reflect acetamidomethylation of protein Cys residues with iodoacetamide
  • Avoid peptides with C-terminal KK, RR, KR or RK (ragged-ends)
  • Avoid peptides containing RP (missed cleavage sites)
  • Avoid hydrophobic sequences as they can be difficult to synthesise or have poor solubility
  • Avoid peptides containing sites for post-translational modifications
  • Check the peptide is unique to your protein (BLAST)