We are often asked by customers, “how successful are your antibodies?” and our general reply is that many of our customers are core antibody groups within large pharmaceutical companies (in-house antibody specialists with many years of generating antibodies themselves) and they have chosen CRB to custom generate their antibodies for more than ten years. So from this we deduce we must have a good success rate and be easy to work with.
Cambridge Research Biochemicals did produce commercial antibodies from 1985 until 1994 when the catalogue business was sold to Sigma-Genosys and therefore any searches made on Cambridge Research Biochemicals will bring up lots of commercial antibodies used and cited in that period.
From 1994 onwards the company focused on custom produced antibodies (mainly against surrogate peptides). However, since most of CRB’s customer base is large Pharma, the number of publications using our antibodies is small.
Here are some examples of CRB-produced antibodies in recent years which have been published and kindly notified to us by customers:
- Mitochondria-derived ROS activate AMP-activated protein kinase (AMPK) indirectly
Murphy, M. et al, MRC Mitochondrial Biology Unit, University of Cambridge (2018) Journal of Biological Chemistry, pp.jbc.RA118.002579.
- Structural basis for membrane tethering by a bacterial dynamin-like pair.
Liu, J., Noel, J. and Low, H., Imperial College London (2018). Nature Communications, 9(1).
- g-TuRC Heterogeneity Revealed by Analysis of Mozart1
Conduit, P. et al., University of Cambridge (2018). Current Biology, 28(14), pp.2314-2323.e6.
- Gene expression of AvBD6-10 in broiler chickens is independent of AvBD6, 9, and 10 peptide potency.
Hall, J. et al., Newcastle University (2018). Veterinary Immunology and Immunopathology, 202, pp.31-40.
- Murine leukemia virus p12 tethers the capsid-containing pre-integration complex to chromatin by binding directly to host nucleosomes in mitosis.
Bishop, K. et al., Francis Crick Institute London (2018). PLOS Pathogens, 14(6), p.e1007117.
- Bioinformatic analysis of meningococcal Msf and Opc to inform vaccine antigen design.
Andreae, C. et al., University of Bristol (2018). PLOS ONE, 13(3), p.e0193940.
- The integrated stress response regulates BMP signalling through effects on translation.
Marciniak, S. et al., University of Cambridge MRC (2018). BMC Biology, 16(1).
- NADH Shuttling Couples Cytosolic Reductive Carboxylation of Glutamine with Glycolysis in Cells with Mitochondrial Dysfunction.
Frezza, C. et al., University of Cambridge MRC (2018). Molecular Cell, 69(4), pp.581-593.e7.
- Evaluation of a novel antibody to define histone 3.3 G34R mutant brain tumours.
Layfield, R. et al., University of Nottingham (2017). Acta Neuropathologica Communications, 5(1).
Anti-Histone H3 (G34R) antibody (crb2005185d)
- Vaccinia virus A11 is required for membrane rupture and viral membrane assembly.
Pénard, E. et al, Centre for Innovation and Technological Research, Ultrapole, Ultrastructural Bio-imaging, Paris, France (2017). Cellular Microbiology, 19(10), p.e12756.
- “Analysis of the Interplay of protein biogenesis factors at the ribosome exit site now reveals new role for NAC.”
Yvonne Nyathi and Martin R. Pool, University of Manchester, JCB, 2015, vol. 210, no. 2, pp. 287-301.
- “Multigene manipulation of photosynthetic carbon assimilation increases CO2 fixation and biomass yield in tobacco”
Christine Raines et al, University of Essex, Journal of Experimental Botony, 2015, 66 (13), pp. 4075-4090
- “Generation of functionally distinct isoforms of PTBP3 by alternative splicing and translation initiation”
Whitfield et al, University of Cambridge & The Babraham Institute, Nucleic Acids Res. 2015, 43 (11), pp. 5586-600.
- “Nek5 promotes centrosome integrity in interphase and loss of centrosome cohesion in mitosis”
Andrew Fry et al, University of Leicester, J. Cell. Biol. 2015, Vol. 209, No. 3, pp.339-348
- “Analysis of the redox oscillations in the circadian clockwork”
John S. O’Neill et al, MRC-Laboratory of Molecular Biology, Methods Enzymol. 2015; 552: p.185-210.
Anti-Peroxiredoxin SO2/3 antibody (crb2005004)
- “Osteoactivin Induces Transdifferentiation of C2C12 Myoblasts into Osteoblasts”
Gregory R Sondag et al, Northeast Ohio Medical University, J. Cell. Physiol. 2014; Vol. 229, Iss. 7, pp. 955-966.
- “LeoA, B and C from Enterotoxigenic Escherichia coli (ETEC) Are Bacterial Dynamins”
Jan Löwe et al, Structural Studies Division-MRC Laboratory of Molecular Biology, PLOS ONE, Sep 2014, Vol 9, Issue 9, e107211.
- “Heightened immune response to autocitrullinated Porphyromonas gingivalis peptidylarginine deiminase: a potential mechanism for breaching immunologic tolerance in rheumatoid arthritis”
Anne-Marie Quirke et al. The Kennedy Institute of Rheumatology, Ann Rheum Dis, 2014; Vol 73, pp. 263–269.
- “Investigation of soluble and Tm-CTLA-4 isoforms in serum and microvesicles”
Linda Wicker, L. Esposito et al. Journal of Immunology, Jul. 2014 193 : 889-900.
- “Molecular Basis and Regulation of OTULIN-LUBAC Interaction”
David Komander et al, MRC Laboratory of Molecular Biology, Molecular Cell, May 2014, p. 335-348, Vol. 3, No. 54
Anti-OTULIN antibody (crb2005228)
- “Biochemical Analysis of the Plasmodium falciparum Erythrocyte-binding Antigen-175 (EBA175)-Glycophorin-A Interaction: implications for vaccine design”
Gavin J. Wright et al, Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, Nov 2013, The Journal of Biological Chemistry vol. 288, no. 45, pp. 32106–32117
- “Coiled-coil domain containing protein 124 is a novel centrosome and midbody protein that interacts with the Ras-guanine nucleotide exchange factor 1B and is involved in cytokinesis”
Telkoparan P et al, PLoS One. Jul 2013, 19; 8 (7): e69289.
- “OTULIN Antagonizes LUBAC Signalling by Specifically Hydrolyzing Met1-Linked Polyubiquitin”
David Komander et al, MRC Laboratory of Molecular Biology, Cell, Jun. 2013, p. 1312–1326, Vol. 6, No. 153.
- “A Systematic Investigation of Differential Effects of Cell Culture Substrates on the Extent of Artifacts in Single-Molecule Tracking” Needham SR et al, Science and Technology Facilities Council, PLoS ONE, 2012, Issue 7, volume 9: e45655
- “A Role for Rab7 in the Movement of Secretory Granules in Cytotoxic T Lymphocytes”
Gillian M. Griffiths et al. The Cambridge Institute for Medical Research, Traffic, 2011; Vol 12, pp. 902–911
- “Regulation of Insulin Receptor Substrate 1 Pleckstrin Homology Domain by Protein Kinase C: Role of Serine 24 Phosphorylation”,
Jaswinder K. Sethi et al, University of Cambridge, Molecular Endocrinology 20 (8): 1838-185
- “Coordinate Regulation of the Mother Centriole Component Nlp by Nek2 and Plk1 Protein Kinases” Andrew M. Fry et al, University of Leicester, Molecular and cellular biology, Feb. 2005, p. 1309–1324 Vol. 25, No. 4
- Anti-SaAQP1o antibody – “Marine Fish Egg Hydration Is Aquaporin-Mediated”
Joan Cerda et al, SCIENCE, Vol 307, 28 Jan 2005
- Specific anti-PEBP-1 antibodies – “A mouse sperm decapacitation factor receptor is phosphatidylethanolamine-binding protein 1”
Lynn Fraser et al, KCL, Reproduction (2005) 130 497-508
Discovery research is highly confidential and a lot of our clients want us to respect their privacy so we are not allowed to mention who we support. However, if you wish to work with us for the first time and would like some reassurance of our success rate, then we’d be happy to put you in touch with one or two of our long-standing customers who are happy to discuss how it feels working with CRB. Please contact us to sort this out for you.