Andrew McKenzie and his team from the MRC Laboratory of Molecular Biology (LMB) enlisted the expertise of the team at Cambridge Research Biochemicals (CRB) to produce a highly specific antibody to the 2A peptide sequence. 2A was discovered in the foot-and-mouth disease virus (FMDV, a picornavirus) and is used in cloning vectors. The team at MRC… Read more »
Posts By: Sarah Smith
The choice to use a peptide or a recombinant protein as the antigen to raise an antibody can be a difficult decision. Here we have outlined the main points to consider in making your choice easier.
Peptides can be used to make highly specific targeted antibodies.
Here at CRB we have an excellent success rate in raising antibodies to recognise PTMs, gained from our years of expertise and our dedication to each project ensuring the best possible outcomes.
Here at CRB we offer a wide range of hosts for antibody production, and whereas mammalian are often the preferred choice, chickens are also growing in popularity. The production of antibodies in the avian host has several advantages and can be an excellent choice in the right circumstances.
Here we outline some of the main advantages of raising antibodies in chicken.
Alain Kohl’s team at the MRC-University of Glasgow Centre for Virus Research chose to recruit the technical expertise of Cambridge Research Biochemicals (CRB) to raise custom polyclonal antibodies specific to the mosquito born Peribunyaviridae family virus; Cache Valley virus (CVV) as well as to Kairi-virus (KRIV). CVV has a tri-segmented, single-stranded genome with negative or ambi-sense polarity which encode… Read more »
Histone proteins help compact and organise the DNA of the nucleus, they also play key roles in orchestrating gene expression. The four core histones, H2A, H2B, H3 and H4 form a complex around which DNA is wound forming the nucleosome. Between the nucleosome is the internucleosomal DNA which is stabilised by the linker histone H1. Modifications of histones can alter the nature… Read more »