Having trouble seeing this email? View it in your browser instead.

October Newsletter:

Peptide Nucleic Acids: Synthetic analogues of DNA
PNA Arrays: Overcoming the limitations of DNA-based arrays
Testimonial: End user experience

CRB Discovery Logo

Peptide Nucleic Acids

PNA is a synthetic analogue of DNA in which the ribose phosphate backbone has been replaced by a polyamide chain. PNA can be used in the same applications as conventional DNA/RNA but because of its unique backbone has a number of advantages:

  • PNA binds to complementary DNA/RNA with higher affinity and greater specificity than conventional oligonucleotides
  • PNA is more stable than DNA or RNA as they are resistant to nucleases and proteases
  • PNA can be hydridized at lower salt concentrations than DNA or RNA which leads to higher stringency

These properties make PNA ideal reagents for antisense and antigene applications, and as probes for molecular diagnostics and microarrays.


Custom PNA Synthesis

CRB offers custom PNAs synthesised with Panagene’s patented BTS technology. This approach allows the routine production of longer PNA oligomers in higher purity than conventional Boc or Fmoc-based methods.

  • Scale: 50 nmole to gram
  • Length: up to 24 residues routinely. Longer PNAs are not ususally necessary due to the very high binding affinity of PNA for DNA or RNA.
  • Dyes or biotin can be attached to N-terminus, C-terminus.
  • PNA can be supplied conjugated to cell penetrating peptides to promote cellular uptake.
  • Optional Lys or PEG spacers can be incorporated to increase solubility.
  • ISO9001:2000 certified

PNA Guides

Click to download these PNA guides, you will need Adobe Reader to view these files.

PANArray™ miRNA expression profiling kit

PANArray™ HPV Genotyping Chip

Protocol of PNA Telomere probe for FISH

GeneChip© Globin-Reduction Kit Handbook

Co-transfecting plasmid DNA and PNAs™ miRNA inhibitor into mammalian cell lines

Need to know more?

Visit the PNA section on our website

  PAN Arrays

PANArrays™ exploit the excellent stability and specificity of PNA to overcome the limitations of DNA-based arrays.

  • Long shelf life
  • Reliable SNP detection
  • Excellent for long DNA detection

Custom PANArrays™ can be developed for SNP analysis, genotyping, expression profiling, and detection of microrganisms. Using Panagene’s FLASH technology, labelling with a fluorescent dye can be carried out after binding of the DNA to the PNA microarray, saving on the use of expensive dyes and allowing for more efficient PCR.
For further information see www.crbdiscovery.com/shop/PANArrays.php

More PNA-based products

miRNA inhibitors

PNA-based miRNA inhibitors offer numerous advantages over conventional inhibitors:

  • Highly sequence specific
  • No cytotoxicity
  • Resistant to cellular nucleases

CRB offers the complete range of Panagene’s human miRNA inhibitors. Other miRNA inhibitors can be made on request. For further information see www.crbdiscovery.com/shop/miRNA.php

Globin Reduction PNA

CRB offers PNA probes for reducing RNA in whole blood samples. These probes bind tightly to globin RNA preventing globin cRNA synthesis, which leads to improved sensitivity against non-globin cRNA. For further information see: www.crbdiscovery.com/shop/pnaglobinreduction.php

PNA Telomere FISH probes

PNA Telomere FISH probes are useful tools for determining telomere length. PNA-based FISH probes offer fast hybridization and less background noise than conventional DNA-based probes. For further information see: www.crbdiscovery.com/shop/pnatelomere.php


To order your PNA synthesis or to learn more about Panagene’s PNA products go to www.crbdiscovery.com/products/pna or telephone: 01642 567180


Testimonial: Dr Liam Good: Royal Vetinary College, University of London; Senior Lecturer in Pathology and Infectious Diseases.

Liam Good

In our research we use peptide nucleic acids (PNA) as antisense agents in bacterial applications. PNAs are better known for their many applications in mammalian systems, but there are also exciting possibilities to develop antisense antibacterial agents and use gene specific PNAs for bacterial gene function studies or even as diagnostic probes.

PNAs provide nuclease stability and high binding affinity, and these are important features that enable unique applications. When working with bacteria, we find that PNAs show superior cell uptake properties, and uptake can be enhanced by attached peptides. There are a variety of nucleic acid analogues and mimics available, and one advantage with PNA is that the pseudo-peptide backbone allows relatively simple attachment of peptide sequences during synthesis. For several years we have used PNAs and peptide-PNAs obtained from PANAGENE via their UK distributor CRB.

We have always been satisfied with product quality. Also, on several occasions we submitted questions about chemistry and the CRB staff efficiently came back with answers and suggestions from their scientists. For researchers who are interested in using PNA in bacterial or other applications, I strongly recommend CRB as a supplier.

CRB will be exhibiting and attending the following exhibitions

Genesis 2010 More info Genes and Cancer More info

View our full range of products

Visit our website to view our full range of products, we offer products for the following:

Peptides PNA Fluorescent Dyes Immunological

Get in touch