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Lanthanide Labelling:
Europium Chelates for Time-Resolved Fluorescence |
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Spectrum™ Peptides:
CRB offers Custom Peptides labelled with the most
comprehensive set of Fluorescent dyes available |
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Fluorescent Dye Selector:
Our interactive online dye selection wheel
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Cambridge Research Biochemicals (CRB) is pleased to offer a new Custom Labelling service using the QuickAllAssay™ Europium Chelate Protein Labelling Kit to label proteins (antibodies) with Europium chelate for inclusion in to Time-Resolved Fluorescence Energy Transfer (TR-FRET); Quenched Resonance Energy Transfer (QRET) assays and Separation based enhanced time-resolved fluorescence assays.
QuickAllAssay™ kits are a range of complete, ready-to-use reagents for labelling of proteins, antibodies, and small molecules with a Europium chelate.
Ideal for TR FRET (Time-Resolved Fluorescence Resonance Energy Transfer) and QRET (Quenched Resonance Energy Transfer assays) and for separation based enhanced time-resolved fluorescence assays.
QRET assays - CRB is also pleased to offer a number of complete assays kits based on QRET (Quenched Resonance Energy Transfer assays)
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| CRB specialises in the synthesis of fluorescently-labelled peptides and antibodies. With licences from leading dye manufacturers such as Life Technologies (Invitrogen-Molecular Probes), GE Healthcare (Amersham Biosciences), Biosearch Technologies, Licor Biosciences and Atto-Tec, we have the largest selection of dyes available for peptide labelling of any company in the world, enabling us to offer the best dye for your particular application. |
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| Available Proprietary Dyes Include: |
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- Life Technologies Dyes: Alexa Fluor®*, Bodipy®*, Oregon Green®
- GE Healthcare: CyDyes™*
- Atto-Tec: ATTO dyes
- Cyanagen: Chromis dyes
- Biosearch Technologies: CAL Fluor®, Quasar®
- Licor Biosciences: IR Dyes®*
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* Requires payment of a royalty.
Non-proprietary dyes: FAM, TAMRA, EDANS, Mca, FITC |
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| Technical Excellence: |
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- Scalability: 0.5 mg to grams
- Dye can be attached to N-terminus, C-terminus or side-chain of the peptide using amino-, thiol-, or click-reactive derivatives
- Optimised strategy for synthesis of FRET peptides to minimise background fluorescence
- ISO9001:2008 certified
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| Delivery |
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Streamlined operation allows delivery in 3-4 weeks. Most of the commonly used dyes are kept in stock to ensure rapid turnaround.
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Our fluorescent dye selector will take you through a step by step process to help you choose the best dye for your application.
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Use our online fluorescent dye selector at
www.crbdiscovery.com/services/dye-labelling/
which provides a list of suitable dyes matching your desired wavelength and the necessary technical data to enable you to select the optimum dye for your application.
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| We offer dyes compatible with all commonly used excitation sources. The most frequently requested are listed, together with appropriate quencher for FRET applications. If you require a peptide labelled with a dye not listed, please enquire as we have access to many more. |
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* denotes CRB’s customer |
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Alexa Fluor 488 labelled peptide
Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes
Roderick H, Tripathi L, Babirye A, Wang D*, Tripathi J, Urwin PE, Atkinson HJ
Molecular Plant Pathology 2012, 13(8):842-851 |
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Alexa 488, Alexa 547, Atto 647N labelling of mouse EGF and affibodies
Optics clustered to output unique solutions: a multi-laser facility for combined single molecule and ensemble microscopy.
Clarke DT, Botchway SW, Coles BC, Needham SR*, Roberts SK*, Rolfe DJ, Tynan CJ, Ward AD, Webb SE, Yadav R, Zanetti-Domingues L, Martin-Fernandez ML*.
Mol Cell Biol. 2011 Jun;31(11):2241-52 |
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Atto 488 and Alexa 546 labelling of mouse EGF (Epidermal Growth Factor)
Human-EGFR aligned on the plasma membrane adopts key features of Drosophila-EGFR asymmetry.
Tynan CJ, Roberts SK*, Rolfe DJ, Clarke DT, Loeffler HH, Kästner J, Winn MD, Parker PJ, Martin-Fernandez ML*.
Mol Cell Biol. 2011 Jun;31(11):2241-52 |
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Monocyclic β-Lactams Are Selective, Mechanism-Based Inhibitors of Rhomboid Intramembrane Proteases
Pierrat OA*, Strisovsky K, Christova Y, Large J, Ansell K, Bouloc N, Smiljanic E, Freeman M.
ACS Chem Biol. 2011 Apr 15;6(4):325-35 |
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Cy3 and Cy5 labelling of mouse EGF (Epidermal Growth Factor)
Single-Molecule Imaging and Fluorescence Lifetime Imaging Microscopy Show Different Structures for High- and Low-Affinity Epidermal Growth Factor Receptors in A431 Cells
Stephen E.D. Webb, Selene K. Roberts*, Sarah R. Needham*, Christopher J. Tynan, Daniel J. Rolfe, Martyn D. Winn, David T. Clarke, Roger Barraclough, and Marisa L. Martin-Fernandez*.
Biophys J. 2008 February 1; 94(3): 803–819 |
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| Click on an event for further information |
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| Should you require a quotation for any of our custom services, we have a clear cut and easy system that enables you to do so. |
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| At Cambridge Research Biochemicals we have a fast-track quotation system that tailors your requirements, gives you all the information you need and a specific price within 24 hours. You are also given a personal contact so that if you have any further questions you can directly contact one of our approachable representatives who has the technical knowledge to assist you. |
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Go to: www.crbdiscovery.com/quotations |
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Click on the relevant tab and register your details. |
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Once issued with a password log on to our quotation system using your customer account log-in and a quotation will be issued within 24 hours. |
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