Antibodies are produced using optimised immunisation protocols in specially designed barrier containment facilities both in the USA under USDA, NIH, AAALACand OLAW accreditation and in the UK under Home Office guidelines.
Standard protocols typically involve a primary immunisation of immunogen (conjugated peptide or customer supplied protein – see protein immunisation guide), emulsified in complete freunds adjuvant followed at 14 day intervals with boosters in incomplete freunds adjuvant to induce the best possible response. Typical immunisation schedules (a number of different species are available) run for 77 days (11 weeks) but can be tailored to individual researchers specific needs. We will be happy to discuss your requirements in detail – please contact us
The development of the antibody response is monitored by ELISA screening at stages throughout the project to enable schedule amendments if required – such amendments to produce the highest titre antibodies can include changing the adjuvant, altering the dose of immunogen or site of injection with test samples available from 6 weeks.