Glycosylation is a common post-translational modification of proteins in which complex carbohydrates are covalently attached by N- or O-glycosidic bonds to the side chains of amino acids, the most common of which are Asn, Ser and Thr.
Simple model glycopeptides can be synthesised in Fmoc solid phase methods by incorporating a protected glycosylated amino acid into the chain and deprotecting the carbohydrate moiety after cleavage of the peptide from the resin. N- and O-linked glycopeptides containing the following glycosylated amino acids can be readily synthesised:
- AcNH-ß-Glc = 2-acetamido-2 deoxy-ß-D-glucopyranosyl
- AcNH-α-Gal = 2-acetamido-2 deoxy-α-D-galactopyranosyl
For other glycopeptides, please contact Cambridge Research Biochemicals to discuss your requirements.